ELECTROPORATION
\ɪlˌɛktɹə͡ʊpəɹˈe͡ɪʃən], \ɪlˌɛktɹəʊpəɹˈeɪʃən], \ɪ_l_ˌɛ_k_t_ɹ_əʊ_p_ə_ɹ_ˈeɪ_ʃ_ə_n]\
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A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. Some applications of electroporation include introduction of plasmids or foreign DNA into living cells for transfection, fusion of cells to prepare hybridomas, and insertion of proteins into cell membranes.
By DataStellar Co., Ltd
Word of the day
tinctura quininae ammoniata
- A preparation made by dissolving quinin sulphate in alcohol [Br. Ph.].