BUERGER'S DISEASE'S METHOD
\bjˈuːəd͡ʒəz dɪzˈiːzɪz mˈɛθəd], \bjˈuːədʒəz dɪzˈiːzɪz mˈɛθəd], \b_j_ˈuː_ə_dʒ_ə_z d_ɪ_z_ˈiː_z_ɪ_z m_ˈɛ_θ_ə_d]\
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For staining the capsules of bacteria: the smear is fixed in Mueller's fluid, washed in water, then alcohol, and covered with tincture of iodine for from 1 to 3 minutes; it is then again washed in alcohol, dried, stained for 2 to 5 seconds in aniline-water gentian-violet, and finally washed in 2 per cent, salt solution.
By Stedman, Thomas Lathrop
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