PULSED FIELD GEL ELECTROPHORESIS
\pˈʌlzd fˈiːld d͡ʒˈɛl ɪlˌɛktɹə͡ʊfɔːɹˈiːsɪs], \pˈʌlzd fˈiːld dʒˈɛl ɪlˌɛktɹəʊfɔːɹˈiːsɪs], \p_ˈʌ_l_z_d f_ˈiː_l_d dʒ_ˈɛ_l ɪ_l_ˌɛ_k_t_ɹ_əʊ_f_ɔː_ɹ_ˈiː_s_ɪ_s]\
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Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
By DataStellar Co., Ltd
Word of the day
Procollagen Proline Dioxygenase
- mixed-function oxygenase that catalyzes hydroxylation prolyl-glycyl-containing-peptide, usually in protocollagen, hydroxyprolylglycyl-peptide. The enzyme utilizes molecular oxygen with a concomitant oxidative decarboxylation of 2-oxoglutarate to succinate. EC 1.14.11.2.