NUCLEASE PROTECTION ASSAYS
\njˈuːklɪˌe͡ɪs pɹətˈɛkʃən ɐsˈe͡ɪz], \njˈuːklɪˌeɪs pɹətˈɛkʃən ɐsˈeɪz], \n_j_ˈuː_k_l_ɪ__ˌeɪ_s p_ɹ_ə_t_ˈɛ_k_ʃ_ə_n ɐ_s_ˈeɪ_z]\
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Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.
By DataStellar Co., Ltd
