LIGASE CHAIN REACTION
\lˈɪɡe͡ɪs t͡ʃˈe͡ɪn ɹɪˈakʃən], \lˈɪɡeɪs tʃˈeɪn ɹɪˈakʃən], \l_ˈɪ_ɡ_eɪ_s tʃ_ˈeɪ_n ɹ_ɪ__ˈa_k_ʃ_ə_n]\
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A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
By DataStellar Co., Ltd
