IN SITU NICK END LABELLING
\ɪn sˈɪtuː nˈɪk ˈɛnd lˈe͡ɪbə͡lɪŋ], \ɪn sˈɪtuː nˈɪk ˈɛnd lˈeɪbəlɪŋ], \ɪ_n s_ˈɪ_t_uː n_ˈɪ_k ˈɛ_n_d l_ˈeɪ_b_əl_ɪ_ŋ]\
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An in situ method for detecting areas of DNA which are nicked during apoptosis. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA fragmentation.
By DataStellar Co., Ltd
Word of the day
hydromorphic
- [Greek] Structurally adapted to an aquatic environment, as organs of water plants.