FREEZE FRACTURING
\fɹˈiːz fɹˈakt͡ʃəɹɪŋ], \fɹˈiːz fɹˈaktʃəɹɪŋ], \f_ɹ_ˈiː_z f_ɹ_ˈa_k_tʃ_ə_ɹ_ɪ_ŋ]\
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Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
By DataStellar Co., Ltd
Word of the day
Procollagen Proline Dioxygenase
- mixed-function oxygenase that catalyzes hydroxylation prolyl-glycyl-containing-peptide, usually in protocollagen, hydroxyprolylglycyl-peptide. The enzyme utilizes molecular oxygen with a concomitant oxidative decarboxylation of 2-oxoglutarate to succinate. EC 1.14.11.2.