FLUORESCENCE ACTIVATED CELL SORTINGS
\flʊ͡əɹˈɛsəns ˈaktɪvˌe͡ɪtɪd sˈɛl sˈɔːtɪŋz], \flʊəɹˈɛsəns ˈaktɪvˌeɪtɪd sˈɛl sˈɔːtɪŋz], \f_l_ʊə_ɹ_ˈɛ_s_ə_n_s ˈa_k_t_ɪ_v_ˌeɪ_t_ɪ_d s_ˈɛ_l s_ˈɔː_t_ɪ_ŋ_z]\
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Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Proto Oncogene Proteins c erbB 2
- cell surface protein-tyrosine kinase that is found to be overexpressed in significant number adenocarcinomas. It has extensive homology can heterodimerize EGF EPIDERMAL GROWTH FACTOR), 3 receptor (RECEPTOR, 3) and the 4 receptor. Activation of erbB-2 receptor occurs during heterodimer formation with a ligand-bound erbB family members. EC 2.7.11.-.