FLUORESCENCE ACTIVATED CELL SORTING
\flʊ͡əɹˈɛsəns ˈaktɪvˌe͡ɪtɪd sˈɛl sˈɔːtɪŋ], \flʊəɹˈɛsəns ˈaktɪvˌeɪtɪd sˈɛl sˈɔːtɪŋ], \f_l_ʊə_ɹ_ˈɛ_s_ə_n_s ˈa_k_t_ɪ_v_ˌeɪ_t_ɪ_d s_ˈɛ_l s_ˈɔː_t_ɪ_ŋ]\
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Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
By DataStellar Co., Ltd
Word of the day
tinctura quininae ammoniata
- A preparation made by dissolving quinin sulphate in alcohol [Br. Ph.].