\ˈankəd pˌiːsˌiːˈɑː], \ˈankəd pˌiːsˌiːˈɑː], \ˈa_n_k_ə_d p_ˌiː_s_ˌiː__ˈɑː]\
Definitions of ANCHORED PCR
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In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Word of the day
beta Aspartic Acid
- An residue in chains that linked at beta-group instead normal, alpha-carboxyl group, polypeptide linkage. It is a result of the spontaneous decomposition aspartic acid or ASPARAGINE residues.